Over 100 fish were killed in a pollution incident in Groudle River during TT week, the gov has said.
Manx Utilities and DEFA investigated and the source was identified as a surface water drain, which serves a nearby housing estate.
However, it is still not clear what killed the fish despite samples being taken and tested.
In total, 110 brown trout were found dead in a 200m stretch of river, directly downstream of the pipe and DEFA continue to monitor the area.
The gov said: ‘People are asked to take extra care of what they dispose of down drains, sinks and toilets after a pollution incident killed more than 100 fish in Groudle Glen during TT week.
‘Surface water drains direct water away from places such as roofs, roads and pathways so should never be used to dispose of things like chemicals, paint or bleach.’